Research Theme 05

Phosphoinositides and Lipid Signalling

Understanding how the acyltransferase LCLAT1 controls the acyl chain composition of phosphoinositide lipids, and how this shapes membrane traffic and cancer cell physiology.

The regulated phosphorylation of phosphatidylinositol (PI) gives rise to seven different species of phosphoinositide (PIP) lipids. PIPs are key regulators of membrane traffic and cellular signaling. PI and PIPs are unique among other phospholipids as they exhibit a remarkable specificity of acyl chains, such that 50–70% of PI/PIPs have an 18:0/20:4 acyl chain profile.

Recent work indicated that the acyltransferase LCLAT1 regulates the incorporation of 18:0 into the sn-1 position of PIPs, and thus contributes to the acyl profile specificity of PIPs.

Collaboration with Dr. Roberto Botelho In collaboration with Dr. Roberto Botelho, we resolved how PIP acyl chains regulated by LCLAT1 (also known as LYCAT) controls the levels, localization, and function of specific PIPs (Bone et al. MBoC 2017).

Current Focus

We are now focused on understanding how LCLAT1 controls the levels and localization of specific PIP species such as PIP2 and PI3P (Chan V et al. MBoC 2024). We aim to understand how the control of PIP species by LCLAT1 can impact growth, proliferation, survival, and invasion in certain types of cancer cells.